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rrid pluripotency undifferentiation markers mouse anti oct4  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rrid pluripotency undifferentiation markers mouse anti oct4
    Rrid Pluripotency Undifferentiation Markers Mouse Anti Oct4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 3466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pluripotency+markers+oct4/Oct-3%2F4+Antibody/pm41512733-67-9-16
    Average 96 stars, based on 3466 article reviews
    rrid pluripotency undifferentiation markers mouse anti oct4 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Knock-Out:

    Article Title: Non-invasive quantification of stem cell-derived islet graft size and composition
    Article Snippet: EC = enterochromaffin -like cells 0 25 50 75 100 M6 Pe rc en t o f a ll ce lls Beta Alpha Delta EC Exocrine Ductal Stroma NA c KO 1G WT 2D WT NT KI Het 2E KI Het 5A KI WT 2H WT NT H2O KI Het 2E KI Het 5A KI WT 2H WT NT PciI Restriction 1500 500 WT 5A 2EWT Knockout WT 5A 2EWT Knockout O CT 4 DN A SS EA 4 DN A TR A 1- 60 D NA d b a Knockout editing Knockin editing Knockin editingKnockout editing ESM Figure 2, quality control of KCNJ11 knockout and R201H knockin editing: a) PCR of the KCNJ11 locus of the knockouts (left) and of the knocked-in area containing PciI restriction site (right) b) Sanger sequencing of the knockin clones c) G-band karyotyping of the knockout and knockin clones d) Immunohistochemisty for pluripotency markers OCT4 (Santa Cruz, sc-9081, 1:250), SSEA4 (Thermo Fisher, MA1021-D488, 1:100) and TRA 1-60 (Thermo Fisher, MA1-023, 1:100) in the knockout and knockin clones a c d e KCNJ11+/+KCNJ11-/- KCNJ11+/R201H Ex am pl e gr af t 2 Ex am pl e gr af t 1 SYP INS DNA b f ESM Figure 3, composition data displayed by purity category and genotype: a) Example immunohistochemistry of pure 5-month SC-islet grafts of different genotypes, stained for endocrine marker synaptophysin (SYP), SC-beta cell marker insulin (INS) and nuclear marker hoechst, scale bar 100 μm b) Implanted SC-islet volume and actual graft volume (including cysts) at 5-months post-implantation c) Cyst proportion of 5-month grafts d) Quantifications of immunohistochemisty, percentage of SYP+ served as basis for categorisation to “pure” and “impure”, cutoff at 50%.

    Knock-In:

    Article Title: Non-invasive quantification of stem cell-derived islet graft size and composition
    Article Snippet: EC = enterochromaffin -like cells 0 25 50 75 100 M6 Pe rc en t o f a ll ce lls Beta Alpha Delta EC Exocrine Ductal Stroma NA c KO 1G WT 2D WT NT KI Het 2E KI Het 5A KI WT 2H WT NT H2O KI Het 2E KI Het 5A KI WT 2H WT NT PciI Restriction 1500 500 WT 5A 2EWT Knockout WT 5A 2EWT Knockout O CT 4 DN A SS EA 4 DN A TR A 1- 60 D NA d b a Knockout editing Knockin editing Knockin editingKnockout editing ESM Figure 2, quality control of KCNJ11 knockout and R201H knockin editing: a) PCR of the KCNJ11 locus of the knockouts (left) and of the knocked-in area containing PciI restriction site (right) b) Sanger sequencing of the knockin clones c) G-band karyotyping of the knockout and knockin clones d) Immunohistochemisty for pluripotency markers OCT4 (Santa Cruz, sc-9081, 1:250), SSEA4 (Thermo Fisher, MA1021-D488, 1:100) and TRA 1-60 (Thermo Fisher, MA1-023, 1:100) in the knockout and knockin clones a c d e KCNJ11+/+KCNJ11-/- KCNJ11+/R201H Ex am pl e gr af t 2 Ex am pl e gr af t 1 SYP INS DNA b f ESM Figure 3, composition data displayed by purity category and genotype: a) Example immunohistochemistry of pure 5-month SC-islet grafts of different genotypes, stained for endocrine marker synaptophysin (SYP), SC-beta cell marker insulin (INS) and nuclear marker hoechst, scale bar 100 μm b) Implanted SC-islet volume and actual graft volume (including cysts) at 5-months post-implantation c) Cyst proportion of 5-month grafts d) Quantifications of immunohistochemisty, percentage of SYP+ served as basis for categorisation to “pure” and “impure”, cutoff at 50%.

    Control:

    Article Title: Non-invasive quantification of stem cell-derived islet graft size and composition
    Article Snippet: EC = enterochromaffin -like cells 0 25 50 75 100 M6 Pe rc en t o f a ll ce lls Beta Alpha Delta EC Exocrine Ductal Stroma NA c KO 1G WT 2D WT NT KI Het 2E KI Het 5A KI WT 2H WT NT H2O KI Het 2E KI Het 5A KI WT 2H WT NT PciI Restriction 1500 500 WT 5A 2EWT Knockout WT 5A 2EWT Knockout O CT 4 DN A SS EA 4 DN A TR A 1- 60 D NA d b a Knockout editing Knockin editing Knockin editingKnockout editing ESM Figure 2, quality control of KCNJ11 knockout and R201H knockin editing: a) PCR of the KCNJ11 locus of the knockouts (left) and of the knocked-in area containing PciI restriction site (right) b) Sanger sequencing of the knockin clones c) G-band karyotyping of the knockout and knockin clones d) Immunohistochemisty for pluripotency markers OCT4 (Santa Cruz, sc-9081, 1:250), SSEA4 (Thermo Fisher, MA1021-D488, 1:100) and TRA 1-60 (Thermo Fisher, MA1-023, 1:100) in the knockout and knockin clones a c d e KCNJ11+/+KCNJ11-/- KCNJ11+/R201H Ex am pl e gr af t 2 Ex am pl e gr af t 1 SYP INS DNA b f ESM Figure 3, composition data displayed by purity category and genotype: a) Example immunohistochemistry of pure 5-month SC-islet grafts of different genotypes, stained for endocrine marker synaptophysin (SYP), SC-beta cell marker insulin (INS) and nuclear marker hoechst, scale bar 100 μm b) Implanted SC-islet volume and actual graft volume (including cysts) at 5-months post-implantation c) Cyst proportion of 5-month grafts d) Quantifications of immunohistochemisty, percentage of SYP+ served as basis for categorisation to “pure” and “impure”, cutoff at 50%.

    Polymerase Chain Reaction:

    Article Title: Non-invasive quantification of stem cell-derived islet graft size and composition
    Article Snippet: EC = enterochromaffin -like cells 0 25 50 75 100 M6 Pe rc en t o f a ll ce lls Beta Alpha Delta EC Exocrine Ductal Stroma NA c KO 1G WT 2D WT NT KI Het 2E KI Het 5A KI WT 2H WT NT H2O KI Het 2E KI Het 5A KI WT 2H WT NT PciI Restriction 1500 500 WT 5A 2EWT Knockout WT 5A 2EWT Knockout O CT 4 DN A SS EA 4 DN A TR A 1- 60 D NA d b a Knockout editing Knockin editing Knockin editingKnockout editing ESM Figure 2, quality control of KCNJ11 knockout and R201H knockin editing: a) PCR of the KCNJ11 locus of the knockouts (left) and of the knocked-in area containing PciI restriction site (right) b) Sanger sequencing of the knockin clones c) G-band karyotyping of the knockout and knockin clones d) Immunohistochemisty for pluripotency markers OCT4 (Santa Cruz, sc-9081, 1:250), SSEA4 (Thermo Fisher, MA1021-D488, 1:100) and TRA 1-60 (Thermo Fisher, MA1-023, 1:100) in the knockout and knockin clones a c d e KCNJ11+/+KCNJ11-/- KCNJ11+/R201H Ex am pl e gr af t 2 Ex am pl e gr af t 1 SYP INS DNA b f ESM Figure 3, composition data displayed by purity category and genotype: a) Example immunohistochemistry of pure 5-month SC-islet grafts of different genotypes, stained for endocrine marker synaptophysin (SYP), SC-beta cell marker insulin (INS) and nuclear marker hoechst, scale bar 100 μm b) Implanted SC-islet volume and actual graft volume (including cysts) at 5-months post-implantation c) Cyst proportion of 5-month grafts d) Quantifications of immunohistochemisty, percentage of SYP+ served as basis for categorisation to “pure” and “impure”, cutoff at 50%.

    Sequencing:

    Article Title: Non-invasive quantification of stem cell-derived islet graft size and composition
    Article Snippet: EC = enterochromaffin -like cells 0 25 50 75 100 M6 Pe rc en t o f a ll ce lls Beta Alpha Delta EC Exocrine Ductal Stroma NA c KO 1G WT 2D WT NT KI Het 2E KI Het 5A KI WT 2H WT NT H2O KI Het 2E KI Het 5A KI WT 2H WT NT PciI Restriction 1500 500 WT 5A 2EWT Knockout WT 5A 2EWT Knockout O CT 4 DN A SS EA 4 DN A TR A 1- 60 D NA d b a Knockout editing Knockin editing Knockin editingKnockout editing ESM Figure 2, quality control of KCNJ11 knockout and R201H knockin editing: a) PCR of the KCNJ11 locus of the knockouts (left) and of the knocked-in area containing PciI restriction site (right) b) Sanger sequencing of the knockin clones c) G-band karyotyping of the knockout and knockin clones d) Immunohistochemisty for pluripotency markers OCT4 (Santa Cruz, sc-9081, 1:250), SSEA4 (Thermo Fisher, MA1021-D488, 1:100) and TRA 1-60 (Thermo Fisher, MA1-023, 1:100) in the knockout and knockin clones a c d e KCNJ11+/+KCNJ11-/- KCNJ11+/R201H Ex am pl e gr af t 2 Ex am pl e gr af t 1 SYP INS DNA b f ESM Figure 3, composition data displayed by purity category and genotype: a) Example immunohistochemistry of pure 5-month SC-islet grafts of different genotypes, stained for endocrine marker synaptophysin (SYP), SC-beta cell marker insulin (INS) and nuclear marker hoechst, scale bar 100 μm b) Implanted SC-islet volume and actual graft volume (including cysts) at 5-months post-implantation c) Cyst proportion of 5-month grafts d) Quantifications of immunohistochemisty, percentage of SYP+ served as basis for categorisation to “pure” and “impure”, cutoff at 50%.

    Clone Assay:

    Article Title: Non-invasive quantification of stem cell-derived islet graft size and composition
    Article Snippet: EC = enterochromaffin -like cells 0 25 50 75 100 M6 Pe rc en t o f a ll ce lls Beta Alpha Delta EC Exocrine Ductal Stroma NA c KO 1G WT 2D WT NT KI Het 2E KI Het 5A KI WT 2H WT NT H2O KI Het 2E KI Het 5A KI WT 2H WT NT PciI Restriction 1500 500 WT 5A 2EWT Knockout WT 5A 2EWT Knockout O CT 4 DN A SS EA 4 DN A TR A 1- 60 D NA d b a Knockout editing Knockin editing Knockin editingKnockout editing ESM Figure 2, quality control of KCNJ11 knockout and R201H knockin editing: a) PCR of the KCNJ11 locus of the knockouts (left) and of the knocked-in area containing PciI restriction site (right) b) Sanger sequencing of the knockin clones c) G-band karyotyping of the knockout and knockin clones d) Immunohistochemisty for pluripotency markers OCT4 (Santa Cruz, sc-9081, 1:250), SSEA4 (Thermo Fisher, MA1021-D488, 1:100) and TRA 1-60 (Thermo Fisher, MA1-023, 1:100) in the knockout and knockin clones a c d e KCNJ11+/+KCNJ11-/- KCNJ11+/R201H Ex am pl e gr af t 2 Ex am pl e gr af t 1 SYP INS DNA b f ESM Figure 3, composition data displayed by purity category and genotype: a) Example immunohistochemistry of pure 5-month SC-islet grafts of different genotypes, stained for endocrine marker synaptophysin (SYP), SC-beta cell marker insulin (INS) and nuclear marker hoechst, scale bar 100 μm b) Implanted SC-islet volume and actual graft volume (including cysts) at 5-months post-implantation c) Cyst proportion of 5-month grafts d) Quantifications of immunohistochemisty, percentage of SYP+ served as basis for categorisation to “pure” and “impure”, cutoff at 50%.

    Immunohistochemistry:

    Article Title: Non-invasive quantification of stem cell-derived islet graft size and composition
    Article Snippet: EC = enterochromaffin -like cells 0 25 50 75 100 M6 Pe rc en t o f a ll ce lls Beta Alpha Delta EC Exocrine Ductal Stroma NA c KO 1G WT 2D WT NT KI Het 2E KI Het 5A KI WT 2H WT NT H2O KI Het 2E KI Het 5A KI WT 2H WT NT PciI Restriction 1500 500 WT 5A 2EWT Knockout WT 5A 2EWT Knockout O CT 4 DN A SS EA 4 DN A TR A 1- 60 D NA d b a Knockout editing Knockin editing Knockin editingKnockout editing ESM Figure 2, quality control of KCNJ11 knockout and R201H knockin editing: a) PCR of the KCNJ11 locus of the knockouts (left) and of the knocked-in area containing PciI restriction site (right) b) Sanger sequencing of the knockin clones c) G-band karyotyping of the knockout and knockin clones d) Immunohistochemisty for pluripotency markers OCT4 (Santa Cruz, sc-9081, 1:250), SSEA4 (Thermo Fisher, MA1021-D488, 1:100) and TRA 1-60 (Thermo Fisher, MA1-023, 1:100) in the knockout and knockin clones a c d e KCNJ11+/+KCNJ11-/- KCNJ11+/R201H Ex am pl e gr af t 2 Ex am pl e gr af t 1 SYP INS DNA b f ESM Figure 3, composition data displayed by purity category and genotype: a) Example immunohistochemistry of pure 5-month SC-islet grafts of different genotypes, stained for endocrine marker synaptophysin (SYP), SC-beta cell marker insulin (INS) and nuclear marker hoechst, scale bar 100 μm b) Implanted SC-islet volume and actual graft volume (including cysts) at 5-months post-implantation c) Cyst proportion of 5-month grafts d) Quantifications of immunohistochemisty, percentage of SYP+ served as basis for categorisation to “pure” and “impure”, cutoff at 50%.

    Staining:

    Article Title: Non-invasive quantification of stem cell-derived islet graft size and composition
    Article Snippet: EC = enterochromaffin -like cells 0 25 50 75 100 M6 Pe rc en t o f a ll ce lls Beta Alpha Delta EC Exocrine Ductal Stroma NA c KO 1G WT 2D WT NT KI Het 2E KI Het 5A KI WT 2H WT NT H2O KI Het 2E KI Het 5A KI WT 2H WT NT PciI Restriction 1500 500 WT 5A 2EWT Knockout WT 5A 2EWT Knockout O CT 4 DN A SS EA 4 DN A TR A 1- 60 D NA d b a Knockout editing Knockin editing Knockin editingKnockout editing ESM Figure 2, quality control of KCNJ11 knockout and R201H knockin editing: a) PCR of the KCNJ11 locus of the knockouts (left) and of the knocked-in area containing PciI restriction site (right) b) Sanger sequencing of the knockin clones c) G-band karyotyping of the knockout and knockin clones d) Immunohistochemisty for pluripotency markers OCT4 (Santa Cruz, sc-9081, 1:250), SSEA4 (Thermo Fisher, MA1021-D488, 1:100) and TRA 1-60 (Thermo Fisher, MA1-023, 1:100) in the knockout and knockin clones a c d e KCNJ11+/+KCNJ11-/- KCNJ11+/R201H Ex am pl e gr af t 2 Ex am pl e gr af t 1 SYP INS DNA b f ESM Figure 3, composition data displayed by purity category and genotype: a) Example immunohistochemistry of pure 5-month SC-islet grafts of different genotypes, stained for endocrine marker synaptophysin (SYP), SC-beta cell marker insulin (INS) and nuclear marker hoechst, scale bar 100 μm b) Implanted SC-islet volume and actual graft volume (including cysts) at 5-months post-implantation c) Cyst proportion of 5-month grafts d) Quantifications of immunohistochemisty, percentage of SYP+ served as basis for categorisation to “pure” and “impure”, cutoff at 50%.

    Marker:

    Article Title: Non-invasive quantification of stem cell-derived islet graft size and composition
    Article Snippet: EC = enterochromaffin -like cells 0 25 50 75 100 M6 Pe rc en t o f a ll ce lls Beta Alpha Delta EC Exocrine Ductal Stroma NA c KO 1G WT 2D WT NT KI Het 2E KI Het 5A KI WT 2H WT NT H2O KI Het 2E KI Het 5A KI WT 2H WT NT PciI Restriction 1500 500 WT 5A 2EWT Knockout WT 5A 2EWT Knockout O CT 4 DN A SS EA 4 DN A TR A 1- 60 D NA d b a Knockout editing Knockin editing Knockin editingKnockout editing ESM Figure 2, quality control of KCNJ11 knockout and R201H knockin editing: a) PCR of the KCNJ11 locus of the knockouts (left) and of the knocked-in area containing PciI restriction site (right) b) Sanger sequencing of the knockin clones c) G-band karyotyping of the knockout and knockin clones d) Immunohistochemisty for pluripotency markers OCT4 (Santa Cruz, sc-9081, 1:250), SSEA4 (Thermo Fisher, MA1021-D488, 1:100) and TRA 1-60 (Thermo Fisher, MA1-023, 1:100) in the knockout and knockin clones a c d e KCNJ11+/+KCNJ11-/- KCNJ11+/R201H Ex am pl e gr af t 2 Ex am pl e gr af t 1 SYP INS DNA b f ESM Figure 3, composition data displayed by purity category and genotype: a) Example immunohistochemistry of pure 5-month SC-islet grafts of different genotypes, stained for endocrine marker synaptophysin (SYP), SC-beta cell marker insulin (INS) and nuclear marker hoechst, scale bar 100 μm b) Implanted SC-islet volume and actual graft volume (including cysts) at 5-months post-implantation c) Cyst proportion of 5-month grafts d) Quantifications of immunohistochemisty, percentage of SYP+ served as basis for categorisation to “pure” and “impure”, cutoff at 50%.



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